Project title:

Application of Nanobiotechnology
for Nutritional Supplementation
with Selenium



Project number:


Project duration:

1. 1. 2019. – 31. 12. 2022.

Funded by:

Croatian Science

Home institution:

Faculty of Agrobiotechnical
Sciences Osijek

Project leader:

PhD. Tomislav Vinković, associate professor

Faculty of Agrobiotechnical Sciences Osijek
Department of Plant Production and Biotechnology

Vladimira Preloga 1 | 31000 Osijek – Croatia
Tel: +385 31 554 889 | Mob: +385 91 224 2055

Collaborating Institutions:

of Agrobiotechnical Sciences

Institute for
Medical Research and
Occupational Health

Faculty of
Pharmacy and

Department of Biology – University of Josip Juraj Strossmayer

Helmholtz Zentrum München: German Research Center for Environmental Health

Project summary:

Selenium (Se) is an essential trace element playing important role in metabolism and many other vital functions. Due to its protective activity in immune response and cancer, it is widely used as a diet supplement.

As plants are the main dietary source of Se for humans and animals, different strategies such as biofortification and plant-based extracts are used to develop Se dietary supplements.

Selenium bioavailability varies depending on several factors including chemical form of Se, other dietary components, physiological status and selenium status of the organism.

The major obstacles in achieving optimal Se intake are highly variable Se content in different food types and narrow safety margin of supplemental Se forms. Nanotechnology enables application of selenium nanoparticles (SeNPs), due to their enhanced antioxidant activities and lower toxicity as compared to other Se forms.

NutriNTENSe project aims to investigate the efficacy and safety of innovative SeNPs-based nutraceuticals and functional food using nanobiotechnological tools.

This will be achived by following development of two innovative classes of Se-nutraceuticals: functional SeNPs biofortified vegetables (FBVegs) and Se-nanoceuticals (SeNCes) using food waste extracts for biogenic synthesis of SeNPs.

Both types of Se-nutraceuticals will be subjected to in vitro and/or in vivo testings to investigate their biocompatibility, safety, pharmacokinetic properties and pharmacological activity compared to selenite and chemically synthesized SeNPs.

In addition, possible synergistic action of biogenic SeNPs and specific bioactive compounds found in NutriNTENSe plants and extracts will be evaluated.

Results obtained within NutriNTENse will be of great interest for consumers, agricultural producers and food industry as final beneficiaries of project outcomes.

It should be highlighted that NutriNTENse is green project aimed to increase implementation of healthy and environment friendly products.